Search results for "Tuberculosis diagnosis"

showing 9 items of 9 documents

Boosting effect of IL-7 in interferon gamma release assays to diagnose Mycobacterium tuberculosis infection.

2018

BACKGROUND A quarter of the world's population is estimated to be infected with Myobacterium tuberculosis (Mtb). Infection is detected by immune response to M. tuberculosis antigens using either tuberculin skin test (TST) and interferon gamma release (IGRA's), tests which have low sensitivity in immunocompromised. IL-7 is an important cytokine for T-cell function with potential to augment cytokine release in in-vitro assays. This study aimed to determine whether the addition of IL-7 in interferon-gamma release assays (IGRAs) improves its diagnostic performance of Mtb infection. METHODS 44 cases with confirmed TB and 45 household contacts without TB were recruited and 1ml of blood was stimul…

0301 basic medicineAdultMaleTuberculosisT-LymphocytesPopulationlcsh:MedicineTuberculin610 Medicine & healthEnzyme-Linked Immunosorbent AssayMycobacterium tuberculosis03 medical and health sciencesInterferon-gammaTuberculosis diagnosisAntigen360 Social problems & social servicesmedicineHumansTuberculosisInterferon gammalcsh:Scienceeducation610 Medicine & healtheducation.field_of_studyAntigens BacterialMultidisciplinarybiologybusiness.industryTuberculin TestInterleukin-7lcsh:RMycobacterium tuberculosisbiology.organism_classificationmedicine.diseaseChemokine CXCL10030104 developmental biologyImmunologylcsh:QFemaleInterferon-gamma Release Testsbusiness360 Social problems & social servicesInterferon-gamma Release Testsmedicine.drugPloS one
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Quantitative and qualitative profiles of circulating monocytes may help identifying tuberculosis infection and disease stages

2017

Tuberculosis (TB) is one of the most important cause of morbidity and death among infectious diseases, and continuous efforts are needed to improve diagnostic tools and therapy. Previous published studies showed that the absolute cells number of monocytes or lymphocytes in peripheral blood or yet the ratio of monocytes to lymphocytes displayed the ability to predict the risk of active TB. In the present study we evaluated the ratio of monocytes to lymphocytes variation and we also analyzed the ex-vivo expression of CD64 on monocytes as tools to identify biomarkers for discriminating TB stages. Significant differences were found when the average ratio of monocytes to lymphocytes of active TB…

0301 basic medicineBacterial DiseasesMalelcsh:MedicineMycobacterium tuberculosiMonocyteMonocytesWhite Blood Cells0302 clinical medicineAnimal CellsMedicine and Health SciencesLymphocyteslcsh:ScienceImmune ResponseAged 80 and overMultidisciplinarybiologyMiddle Aged3. Good healthActinobacteriamedicine.anatomical_structureInfectious DiseasesPhenotypeAdolescent; Adult; Aged; Aged 80 and over; Biomarkers; Case-Control Studies; Female; Humans; Male; Middle Aged; Monocytes; Mycobacterium tuberculosis; Phenotype; Tuberculosis; Young Adult; Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)Biomarker (medicine)Tuberculosis Diagnosis and ManagementFemaleCellular TypesCase-Control StudieResearch ArticleHumanAdultTuberculosisAdolescentTuberculosiImmune CellsImmunologyMycobacterium tuberculosis03 medical and health sciencesYoung AdultTuberculosis diagnosisDiagnostic MedicinemedicineHumansTuberculosisAgedBlood CellsBiochemistry Genetics and Molecular Biology (all)Receiver operating characteristicBacteriabusiness.industryMonocytelcsh:RCase-control studyOrganismsBiology and Life SciencesMycobacterium tuberculosisCell BiologyBiomarkerbiology.organism_classificationmedicine.diseaseTropical DiseasesConfidence interval030104 developmental biologyAgricultural and Biological Sciences (all)Case-Control StudiesImmunologylcsh:QbusinessBiomarkers030215 immunology
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Identification of plasma biomarkers for discrimination between tuberculosis infection/disease and pulmonary non tuberculosis disease.

2018

We used the Luminex Bead Array Multiplex Immunoassay to measure cytokines, chemokines and growth factors responses to the same antigens used for RD1-based Interferon γ Release Assay (IGRA) test. Seventy-nine individuals, 27 active TB, 32 latent infection subsets, 20 individuals derivative purified protein (PPD) negative (subjects that do not have any indurative cutaneous reaction after 72 hrs of intradermal injection of PPD) and with other pulmonary disease were retrospectively studied. Forty-eight analytes were evaluated by Luminex Assay in plasma obtained from whole blood stimulated cells. The diagnostic accuracies of the markers detected were evaluated by ROC curve analysis and by the co…

0301 basic medicineMaleBacterial DiseasesPhysiologylcsh:MedicineBiochemistry0302 clinical medicineBiochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)Immune PhysiologyBlood plasmaMedicine and Health SciencesMultiplex030212 general & internal medicinelcsh:ScienceWhole bloodInnate Immune SystemMultidisciplinarymedicine.diagnostic_testLatent tuberculosisbiologyChemotaxis3. Good healthBody FluidsActinobacteriaCell MotilityInfectious DiseasesBloodCytokinesTuberculosis Diagnosis and ManagementChemokinesAnatomyResearch ArticleAdultTuberculosisSettore MED/17 - Malattie InfettiveImmunologyBlood PlasmaMycobacterium tuberculosis03 medical and health sciencesAntigenLatent TuberculosisDiagnostic MedicinemedicineSettore MED/05 - Patologia ClinicaHumansTuberculosisTuberculosis PulmonaryAntigens BacterialBiochemistry Genetics and Molecular Biology (all)Bacteriabusiness.industrylcsh:ROrganismsBiology and Life SciencesMycobacterium tuberculosisCell BiologyMolecular Developmentmedicine.diseasebiology.organism_classificationTropical Diseases030104 developmental biologyAgricultural and Biological Sciences (all)ImmunoassayImmune SystemImmunologylcsh:QbusinessBiomarkersDevelopmental BiologyPloS one
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Low sensitivity of the MPT64 identification test to detect lineage 5 of the Mycobacterium tuberculosis complex

2018

Abstract: Purpose. Differentiation of the Mycobacterium tuberculosis complex (MTBc) from non-tuberculous mycobacteria (NTM) is important for tuberculosis diagnosis and is a prerequisite for reliable phenotypic drug-resistance testing. We evaluated the performance of the rapid MPT64 antigen identification test for the detection of Mycobacterium africanum lineage 5 (MAF L5). Methodology. Smear-positive tuberculosis patients' sputa were included prospectively. Culture was performed on Lowenstein-Jensen medium and, when positive, the MPT64 test and the classical para-nitro benzoic acid susceptibility and heat-labile catalase (PNB/catalase) identification tests were performed. The MPT64 test was…

0301 basic medicineMicrobiology (medical)TuberculosisRepeat testing030106 microbiologyPolymorphism Single NucleotideSensitivity and SpecificityMicrobiologyMicrobiology03 medical and health sciencesTuberculosis diagnosisAntigenmedicineHumansTuberculosisBiologyAntigens BacterialbiologyGene Expression Regulation BacterialMycobacterium tuberculosisGeneral Medicinebiology.organism_classificationmedicine.diseaseBacterial Typing Techniques3. Good healthMycobacterium tuberculosis complexNonsynonymous snpsMycobacterium africanumJournal of Medical Microbiology
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Performance of a Highly Sensitive Mycobacterium tuberculosis Complex Real-Time PCR Assay for Diagnosis of Pulmonary Tuberculosis in a Low-Prevalence …

2018

ABSTRACT The potential impact of routine real-time PCR testing of respiratory specimens from patients with presumptive tuberculosis in terms of diagnostic accuracy and time to tuberculosis treatment inception in low-prevalence settings remains largely unexplored. We conducted a prospective intervention cohort study. Respiratory specimens from 1,020 patients were examined by acid-fast bacillus smear microscopy, tested by a real-time Mycobacterium tuberculosis complex PCR assay (Abbott RealTi me MTB PCR), and cultured in mycobacterial media. Seventeen patients tested positive by PCR (5 were acid-fast bacillus smear positive and 12 acid-fast bacillus smear negative), and Mycobacterium tubercul…

AdultMale0301 basic medicineMicrobiology (medical)medicine.medical_specialtyTime FactorsTuberculosisAdolescent030106 microbiologyAntitubercular AgentsReal-Time Polymerase Chain ReactionMycobacterium tuberculosisYoung Adult03 medical and health sciencesTuberculosis diagnosisInternal medicineHumansMedicineProspective StudiesTuberculosis PulmonaryAgedAged 80 and overBacteriological TechniquesMicroscopybiologybusiness.industryMycobacteriology and Aerobic ActinomycetesMycobacterium tuberculosisMiddle Agedmedicine.diseasebiology.organism_classificationIntervention studiesReal-time polymerase chain reactionMolecular Diagnostic TechniquesMycobacterium tuberculosis complexFemaleNontuberculous mycobacteriabusinessCohort studyJournal of Clinical Microbiology
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Novel M. tuberculosis specific IL-2 ELISpot assay discriminates adult patients with active or latent tuberculosis

2018

Background Tuberculosis (TB) still is a major worldwide health problem, with 10.4 million new cases in 2016. Only 5–15% of people infected with M. tuberculosis develop TB disease while others remain latently infected (LTBI) during their lifetime. Thus, the absence of tests able to distinguish between latent infection and active tuberculosis is one of the major limits of currently available diagnostic tools. Methods A total of 215 patients were included in the study as active TB cases (n = 73), LTBI subjects (n = 88) and healthy persons (n = 54). Peripheral blood mononuclear cells (PBMCs) were isolated from each patient and the LIOSpot® TB anti-human IL-2 ELISpot assay was performed to test …

Bacterial DiseasesMale0301 basic medicinelcsh:MedicineAdult; Aged; Case-Control Studies; Diagnosis Differential; Female; Humans; Immunoassay; Interleukin-2; Latent Tuberculosis; Male; Middle Aged; Mycobacterium tuberculosis; ROC Curve; Species SpecificityFluorescence MicroscopyBiochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)ZoonosesDiagnosisMedicine and Health SciencesBovine TuberculosisEnzyme-Linked Immunoassayslcsh:ScienceImmunoassayMicroscopyMultidisciplinarybiologyLatent tuberculosismedicine.diagnostic_testELISPOTLight MicroscopyMiddle AgedActinobacteriaInfectious DiseasesTuberculosis Diagnosis and ManagementFemaleResearch ArticleAdultTuberculosis030106 microbiologyResearch and Analysis MethodsQuantiFERONDiagnosis DifferentialMycobacterium tuberculosis03 medical and health sciencesSpecies SpecificityAntigenDiagnostic MedicineLatent TuberculosismedicineTuberculosisHumansImmunoassaysAgedBacteriabusiness.industrylcsh:ROrganismsCase-control studyBiology and Life SciencesMycobacterium tuberculosisTropical Diseasesmedicine.diseasebiology.organism_classificationMycobacterium Ulcerans030104 developmental biologyROC CurveCase-Control StudiesImmunoassayDifferentialImmunologyImmunologic TechniquesInterleukin-2lcsh:QbusinessPLOS ONE
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"Treatment is of primary importance, and social assistance is secondary": A qualitative study on the organisation of tuberculosis (TB) care and patie…

2018

Background Vulnerable individuals with tuberculosis (TB) struggle to access and stay on treatment. While patient-related and social barriers to TB treatment adherence are well documented, less is known about how the organisation and delivery of TB care influences adherence behaviour. Aim To examine the influence of TB service organisation and culture on patients’ experience of starting and staying on treatment in Riga, Latvia. Methods An intervention package to support adherence to TB treatment amongst vulnerable patients in Riga, Latvia was piloted between August 2016 and March 2017. Qualitative observations (5), interviews with staff (20) and with TB patients (10) were conducted mid-way a…

Bacterial DiseasesMaleHealth Knowledge Attitudes PracticeMedical DoctorsEpidemiologyEconomicsHealth Care ProvidersPsychological interventionNursesSocial Scienceslcsh:MedicinePilot ProjectsLiteracy0302 clinical medicineMedicine and Health SciencesMedicineMedical Personnel030212 general & internal medicinelcsh:ScienceQualitative Researchmedia_commonMultidisciplinaryPharmaceuticsMiddle AgedProfessionsInfectious DiseasesTuberculosis Diagnosis and ManagementFemaleResearch ArticleAdultTuberculosismedia_common.quotation_subject030231 tropical medicineMEDLINE03 medical and health sciencesSocial supportDrug TherapyNursingDiagnostic MedicineIntervention (counseling)TuberculosisHumansbusiness.industrylcsh:RSocial SupportTropical Diseasesmedicine.diseaseLatviaHealth CareMedical Risk FactorsService (economics)People and PlacesPatient CompliancePopulation Groupingslcsh:QbusinessDelivery of Health CareFinanceQualitative researchPLOS ONE
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Where are patients missed in the tuberculosis diagnostic cascade? A prospective cohort study in Ghana.

2020

BackgroundGhana's national prevalence survey showed higher than expected tuberculosis (TB) prevalence, indicating that many people with TB are not identified and treated. This study aimed to identify gaps in the TB diagnostic cascade prior to starting treatment.MethodsA prospective cohort study was conducted in urban and rural health facilities in south-east Ghana. Consecutive patients routinely identified as needing a TB test were followed up for two months to find out if sputum was submitted and/or treatment started. The causal effect of health facility location on submitting sputum was assessed before risk factors were investigated using logistic regression.ResultsA total of 428 persons …

MaleRural PopulationBacterial DiseasesRNA virusesResearch FacilitiesUrban PopulationPhysiologyHIV InfectionsPathology and Laboratory MedicineGhanaCohort StudiesGeographical Locations0302 clinical medicineImmunodeficiency VirusesHealth facilityHealth careOdds RatioMedicine and Health SciencesCoughingProspective Studies030212 general & internal medicineProspective cohort studyMultidisciplinaryRural healthQRMiddle AgedBody FluidsInfectious DiseasesMedical MicrobiologyResearch DesignViral PathogensVirusesTuberculosis Diagnosis and ManagementMedicineFemaleAnatomyPathogensmedicine.symptomResearch LaboratoriesResearch ArticleCohort studyAdultmedicine.medical_specialtyTuberculosisAdolescentScience030231 tropical medicineResearch and Analysis MethodsMicrobiologyYoung Adult03 medical and health sciencesSigns and SymptomsTuberculosis diagnosisDiagnostic MedicineRetrovirusesmedicineHumansTuberculosisMicrobial PathogensAgedbusiness.industryLentivirusSputumOrganismsBiology and Life SciencesHIVTropical Diseasesmedicine.diseaseMucusFamily medicinePeople and PlacesAfricaSputumHealth FacilitiesPhysiological ProcessesbusinessGovernment LaboratoriesPLoS ONE
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Whole-genome sequencing of Mycobacterium tuberculosis directly from clinical samples for high-resolution genomic epidemiology and drug resistance sur…

2020

9 páginas, 3 figuras

Microbiology (medical)Whole genome sequencingmedicine.medical_specialtylcsh:R5-920Tuberculosislcsh:QR1-502Single-nucleotide polymorphismDrug resistanceComputational biologyBiologybiology.organism_classificationmedicine.diseaseMicrobiologylcsh:MicrobiologyMycobacterium tuberculosisInfectious DiseasesTuberculosis diagnosisVirologyEpidemiologymedicineSputummedicine.symptomlcsh:Medicine (General)
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